Product Uses Include
Analysis of in vivo RhoA activation levels
Detection of compounds and proteins that enhance RhoA activity
Detection of compounds and proteins that inhibit RhoA activity
The activity of RhoA in cells (tissues) was studied qualitatively
Product Description
| Description | Anti-Active RhoA Mouse Monoclonal Antibody |
| Another Name | Anti-RhoA-GTP Mouse Monoclonal Antibody |
| Catalog Number | NL23002 |
| Size: | 30 µl |
| Tested Applications: | IP , IHF IHC |
| Cited Applications: | IP , IHC , IF , FC ICC ICF IHF |
| Tested Reactivity: | Human , mouse |
| Cited Reactivity: | Human , mouse Rat |
| Gene Symbol: | RhoA |
| Immunogen : | Recombinant full length protein of active RhoA |
| Concentration: | 1mg/ml |
| Host/Isotype: | mouse/IgG2b |
| Class: | monnoclonal |
| Type: | Antibody |
| Predicted mv: | 22KDa |
| Observed mv: | 22KDa |
| Uniprot: | p61586 |
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Conditions: | Store at -20°C for one year after shipment |
|
Background: |
Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
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Tested Applications: IP/WB
Tested Applications: IF
实验方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol |
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
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