Product Description
| Description | Anti-EGFR (vIII) Mouse Monoclonal Antibody |
| Another Name | Anti-EGFR (Mutated vIII) Mouse Monoclonal Antibody |
| Catalog Number | NL25101 |
| Size: | 100 µl |
| Tested Applications: | ELISA WB IF IHC ELISA:1:500-1:1000 WB:1:500-1:1000 IF:1:50-1:200 |
| Cited Applications: | WB IF |
| Tested Reactivity: | Human , mouse Rat |
| Cited Reactivity: | Human , mouse Rat |
| Gene Symbol: | ERBB; HER1; mENA; ERBB1; PIG61; NISBD2 |
| Immunogen : | A synthetic peptide from the internal region of human EGFR which includes the mutation of A839T |
| Concentration: | 1mg/ml |
| Host/Isotype: | mouse/IgG |
| Class: | monnoclonal |
| Type: | Antibody |
| Predicted mv: | 134 KDa |
| Observed mv: | 30 KDa |
| Uniprot: | P00533 |
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Conditions: | Store at -20°C for one year after shipment |
Background: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily.This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylati on and leads to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of coronavirus disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). |
Tested Applications: WB
Tested Applications: IF
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(vIII) plasmid (right column), then fixed and stained with anti-EGFR(vIII) monoclonal antibody (Cat. #NL25101)
实验方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol |
操作指南
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操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
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