Product Description
| Description | Anti-KRas (G12D) Mouse Monoclonal Antibody | 
| Another Name | Anti-KRas (Mutated G12D) Mouse Monoclonal Antibody | 
| Catalog Number | NL25091 | 
| Size: | 100 µl | 
| Tested Applications: | ELISA WB IF ELISA:1:500-1:1000 WB:1:500-1:1000 IF:1:50-1:200 | 
| Cited Applications: | WB IF | 
| Tested Reactivity: | Human , mouse Rat | 
| Cited Reactivity: | Human , mouse Rat | 
| Gene Symbol: | 
 CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; ' C-K-RAS; c-Ki-ras; c-Ki-ras2; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1  | 
| Immunogen : | A synthetic peptide from the internal region of KRas which includes the mutation of G12D, human origin | 
| Concentration: | 1mg/ml | 
| Host/Isotype: | mouse/IgG1,IgG2a ,IgG2b | 
| Class: | monnoclonal | 
| Type: | Antibody | 
| Predicted mv: | 21 KDa | 
| Observed mv: | 21 KDa | 
| Uniprot: | P01116 | 
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | 
| Storage Conditions: | Store at -20°C for one year after shipment | 
| 
 
 Background:  | 
 Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. 
  | 
Tested Applications: WB
                   
Tested Applications: IF
                     
  
实验方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol | 
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol | 
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |