| 品牌： | NEWLF  |
| 价格： | ￥4480.00 |
| 规格： | 100ul |
| 货号： | NL25107 |
| 应用： | ELISA WB IF |
Product Description
| Description | Anti-PIK3CA (E545G) Mouse Monoclonal Antibody |
| Another Name | Anti-PIK3CA (Mutated E545G) Mouse Monoclonal Antibody |
| Catalog Number | NL25107 |
| Size: | 100 µl |
| Tested Applications: | ELISA WB IF IHC ELISA:1:500-1:1000 WB:1:500-1:1000 IF:1:50-1:100 |
| Cited Applications: | WB |
| Tested Reactivity: | Human , mouse Rat |
| Cited Reactivity: | Human , mouse Rat |
| Gene Symbol: | MCM; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha |
| Immunogen : | A synthetic peptide from the internal region of human PIK3CA which includes the mutation of E545G |
| Concentration: | 1mg/ml |
| Host/Isotype: | mouse/IgG |
| Class: | monnoclonal |
| Type: | Antibody |
| Predicted mv: | 124 KDa |
| Observed mv: | 29 KDa |
| Uniprot: | P42336 |
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Conditions: | Store at -20°C for one year after shipment |
Background: | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. |
Tested Applications: WB
Tested Applications: IF
Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti-PIK3CA(E545G) monoclonal antibody (Cat. #NL25107).
实验方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol |
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
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