Product Description
| Description | Anti-KRas (Q61H) Mouse Monoclonal Antibody |
| Another Name | Anti-KRas (Mutated Q61H) Mouse Monoclonal Antibody |
| Catalog Number | NL25094 |
| Size: | 100 µl |
| Tested Applications: | ELISA WB IF ELISA:1:500-1:1000 WB:1:500-1:1000 IF:1:50-1:200 |
| Cited Applications: | WB IF |
| Tested Reactivity: | Human , mouse Rat |
| Cited Reactivity: | Human , mouse Rat |
| Gene Symbol: | CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; ' C-K-RAS; c-Ki-ras; c-Ki-ras2; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1 |
| Immunogen : | A synthetic peptide from the internal region of KRas which includes the mutation of Q61H, human origin |
| Concentration: | 1mg/ml |
| Host/Isotype: | mouse/IgG |
| Class: | monnoclonal |
| Type: | Antibody |
| Predicted mv: | 21 KDa |
| Observed mv: | 21 KDa |
| Uniprot: | P01116 |
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Conditions: | Store at -20°C for one year after shipment |
Background: | Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras Q61H mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
Tested Applications: WB
Tested Applications: IF
Immunofluorescence of cells expressing Ras proteins with Anti-Ras(Q61H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(Q61H) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) plasmid, then fixed and stained with Anti-Ras(Q61H) monoclonal antibody (Cat. #NL25094). 61H) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) , then fixed and stained with Anti-Ras(Q61H) monoclon
实验方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol |
操作指南
| 免疫印迹实验操作指南 Download protocol | |
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| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
操作指南
| 免疫印迹实验操作指南 Download protocol | |
| 免疫组化实验操作指南 Download protocol | |
| 免疫沉淀实验操作指南 Download protocol | |
| 免疫细胞化学实验操作指南 Download protocol | |
| 免疫荧光实验操作指南 Download protocol |
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