Anti-RHEB(Y35N) mouse monoclonal antibody

价格:¥4480.00
品牌:NEWLF   
货号:NL25117
规格:100ul

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    Product  Description


       Description    Anti-RHEB (Y35N) mouse monoclonal antibody
       Another Name    Anti-RHEB (mutated Y35N) mouse monoclonal antibody
       Catalog Number    NL25117
       Size:     100 µl
       Tested Applications:      ELISA  WB  IF  IHC        ELISA:1:500-1:1000       WB:1:500-1:1000      IF:1:50-1:100
       Cited  Applications:    WB   IF
       Tested Reactivity:     Human , mouse   Rat
       Cited  Reactivity:       Human , mouse   Rat
       Gene Symbol:  

        RHEB2

       Immunogen :      A synthetic peptide from the internal region of human RHEB which includes the mutation of Y35N
       Concentration:                1mg/ml
       Host/Isotype:                     mouse/IgG
       Class:                   monnoclonal
       Type:                                  Antibody
       Predicted mv:                     20 KDa
       Observed mv:                 20 KDa
       Uniprot:                              Q15382
       Constituents:      PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
       Storage Conditions:       Store at -20°C for one year after shipment

      

        Background:  

        This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with 

        five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle 

        progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles 

        between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three 

        pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.





    Tested Applications:  WB

          

                            1685525139336.png

                                      

                     

     Tested Applications:  IF


                                8c68b5bbf07fb5fe88feb606e0d3d640_D195535-IF-1-P.png

        

    Immunofluorescence of cells expressing RHEB proteins with Anti RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP RHEB(WT) plasmid (left column) or pCDNA3-GFP RHEB(Y35N) plasmid (right column), then fixed and stained with Anti RHEB(Y35N) monoclonal antibody (Cat. #NL25117).

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